Identification of valid endogenous control genes for determining gene expression in human glioma
نویسندگان
چکیده
منابع مشابه
Identification of valid endogenous control genes for determining gene expression in human glioma.
In human glioma, quantitative real-time reverse-transcription PCR (qPCR) is a frequently used research tool. However, no systematic analysis of suitable reference genes for reliable gene expression analysis has been performed so far. In the current study, we tested 19 commonly used reference genes for their expression stability in human astrocytoma WHO Grade II, astrocytoma WHO Grade III, and g...
متن کاملIdentification of Stably Expressed lncRNAs as Valid Endogenous Controls for Profiling of Human Glioma
BACKGROUND Recent research indicates that long non-coding RNAs (lncRNA) represent a new family of RNAs that is of fundamental importance for controlling transcription and translation. Thereby, there is increasing evidence that lncRNAs are also important in tumourigenesis. Thereby valid expression profiling using quantitative PCR requires suitable, stably expressed normalisers to achieve reliabl...
متن کاملIdentification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172
Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA br...
متن کاملValidation of Endogenous Control Genes for Gene Expression Studies on Human Ocular Surface Epithelium
PURPOSE To evaluate a panel of ten known endogenous control genes (ECG) with quantitative reverse transcription PCR (qPCR), for identification of stably expressed endogenous control genes in the ocular surface (OS) epithelial regions including cornea, limbus, limbal epithelial crypt and conjunctiva to normalise the quantitative reverse transcription PCR data of genes of interest expressed in ab...
متن کاملcomparison of snrna-u6 and microrna-16 for identification of suitable endogenous control gene for microrna gene expression analysis under dendrosomal curcumin treatment in hepatocellular carcinoma cell lines
objective: micrornas (mirnas) are single-stranded small rnas 18-25 nucleotides in length that regulate gene expression through translational inhibition and mrna cleavage. aberrant expression of mirnas contribute to several diseases. this has increased interest in profiling the expressions of these molecules. real-time quantitative pcr (rq-pcr) is a sensitive, quantitative technique for gene exp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Neuro-Oncology
سال: 2010
ISSN: 1522-8517,1523-5866
DOI: 10.1093/neuonc/nop072